Analysing a polyclonal karyotype
Sometimes a few clonal lines can be distinguished from each other during analysis of a case. Analysis of such a polyclonal karyotype is performed by calculating a "composite" ("[cp]") karyotype an dthen performing analysis on this composite karyotype.
Let us assume that the karyotype formulae are entered in the textbox 'txtKaryotype' on a form, separated from each other by a forward slash ("/"). After clicking the button 'Button1', the composite karyotype is calculated, that karyotype is analysed as shown in "Analysing a monoclonal karyotype", and the results are written into the multiline textbox 'txtResult'.
At first, integrate the ISCNAnalyser dll into your project. From the
menu 'Project' select 'Add references'. The ISCNAnalyser
located in its installation directory (normally C:\CyDAS\).
Add an 'Imports' statement in your form before the beginning
of the class:
In the 'Button1_Click' procedure add:
'define a Karyotypes object Dim oKaryos As Karyotypes
'the composite karyotype will be stored herein Dim objKaryotype As Karyotype 'initialise the karyotypes object with the textual description oKaryos = New Karyotypes(txtKaryotype.Text)
'try to get a composite karyotype Try 'query the karyotypes object; it may throw an error, if a karyotype is not valid objKaryotype = oKaryos.getCompositeKaryotype 'since we got here, there was no error and we do really have a composite karyotype 'we pass it to the local analysis function performAnalyisis(objKaryotype) Catch exc As Exception 'an error has occured, show it to the user txtResult.Text &= "Error while constructing composite Karyotype: " & vbCrLf 'the message of the last error txtResult.Text &= exc.Message 'sometimes, there is an inner error If Not exc.InnerException Is Nothing Then txtResult.Text &= vbCrLf & exc.InnerException.Message End If End Try
The analysis of a the composite karyotype is then performed as described in "Analysing a monoclonal karyotype".